MicroMedicine™ Featured in “Improving Immunophenotyping Standardization” Editorial

Variability can be introduced at multiple stages within an immunophenotyping experiment, not least during the initial isolation and concentration of white blood cells (WBC) from whole blood. This is typically performed by density gradient centrifugation, a labor-intensive process that can artificially skew results. “During density gradient centrifugation, cells experience significant centrifugal forces with concurrent exposure to chemical reagents,” notes Anya Manning, senior product manager at MicroMedicine. “This can influence cellular quality or result in the loss of cells before you even begin your assay. Density gradient centrifugation also narrows potential downstream analysis by eliminating polymorphonuclear cells such as granulocytes, retaining only monocytes and lymphocytes for subsequent immunostaining.”

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